What is Genetic Engineering

 

 

 

 

Introduction

 

DNA

Print this page and answer each of the questions using the websites below as well as your textbook These questions will be your study guide to prepare you for your chapter test.

1.  Define and describe the types of selective breeding.  How are mutagens
     used in selective breeding?
     http://en.wikipedia.org/wiki/Artificial_selection

     http://www.learner.org/courses/essential/life/session5/closer1.html
     http://learn.genetics.utah.edu/archive/mutations/index.html

2.  Define  "genetic engineering" and give two examples.
     http://www.psrast.org/whatisge.htm

     http://en.wikipedia.org/wiki/Human_genetic_engineering

3.  One important technique in genetic engineering is the production of 
     recombinant DNA. 
     Define recombinant DNA (rDNA)?
     List and describe the steps used to make rDNA.
    
You may use your book as well as the listed websites to answer the question.
     http://www.rpi.edu/dept/chem-eng/Biotech-Environ/Projects00/rdna/rdna.html

     http://www.newworldencyclopedia.org/entry/Recombinant_DNA
     http://askabiologist.asu.edu/restriction-enzymes
     http://www.stat.berkeley.edu/users/terry/Classes/s260.1998/Week8b/week8b/node3.html
     http://www.iptv.org/exploremore/ge/what/recombinant.cfm
     steps in cloning a gene

4.  Describe three examples of how recombinant DNA is used.
     http://www.iptv.org/exploreMore/ge/uses/use2_medical.cfm
    
5.  How do the terms recombinant DNA, plasmid, and vector apply to genetic
     engineering?
     http://en.wikipedia.org/wiki/Plasmid  
     http://en.wikipedia.org/wiki/Vector_DNA  
     http://www.rpi.edu/dept/chem-eng/Biotech-Environ/Projects00/rdna/rdna.html

6.  What is a polymerase chain reaction.  Name and describe three uses of PCR.
      http://www.dnatube.com/view_video2.php?viewkey=636e4a163a5e3d16adab
      http://en.wikipedia.org/wiki/PCR

7.  DNA fingerprinting is a very important application of genetic engineering.    
     Define DNA fingerprinting and describe the steps involved to make a DNA
     fingerprint.  How are DNA fingerprints used?
     You may use your book as well as the listed websites to answer the question.
     http://www.accessexcellence.org/RC/AB/BA/DNA_Fingerprinting_Basics.php

     http://www.ornl.gov/sci/techresources/Human_Genome/elsi/forensics.shtml

8.  Describe the process of gel electrophoresis and relate it to DNA fingerprinting.
     http://www.sumanasinc.com/webcontent/animations/content/gelelectrophoresis.html
   
       
http://faculty.uca.edu/march/bio1/DNA_GMO/dnaflow.htm
     http://www.biotech.iastate.edu/biotech_info_series/bio6.html
 

9.  What are polymorphisms and restriction fragment length polymorphisms (also
     known as variable tandem repeats)?  What is their importance in DNA
     fingerprinting?
     You may use your book and the following websites to answer the question.
     http://www.scq.ubc.ca/a-brief-tour-of-dna-fingerprinting/
     http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/R/RFLPs.html

10.  What is southern blot analysis and how is it used in DNA fingerprinting?
       http://www.bio.davidson.edu/courses/genomics/method/Southernblot.html
       http://www.molecularstation.com/dna/southern-blot/
       http://highered.mcgraw-hill.com/olcweb/cgi/pluginpop.cgi?
       it=swf::535::535::/sites/dl/free/0072437316/120078/bio_g.swf::Southern+Blot

11
.  Define  "gene therapy" and explain how it works.  Give two beneficial
       examples of how it could be used.  Explain its benefits, risks and challenges.
       http://learn.genetics.utah.edu/content/tech/genetherapy/whatisgt/   
       http://learn.genetics.utah.edu/content/tech/genetherapy/gtdelivery/
       http://learn.genetics.utah.edu/content/tech/genetherapy/gtchallenges/
       http://en.wikipedia.org/wiki/Human_genetic_engineering

12.  One of the futures of medicine is genetically engineered (recombinant vector)
       vaccines.   Explain what they are and their potential benefits.
       http://www.stopgettingsick.com/template.cfm-1702

       http://en.wikipedia.org/wiki/DNA_vaccination

    

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What is Genetic Engineering

 

 

GMO's

 

 

The Big Question

 

 

Teacher Page

 

 

Credits

 

Site designed and maintained by  Pat De Candia
Last updated:  May 17, 2011